In this study, energy and exergy efficiency uncertainties of a large scale lignite fired power plant cycle and various measurement parameter sensitivities were investigated for five different design power outputs (100%, 85%, 80%, 60% and 40%) and with real data of the plant. For that purpose a black box method was employed considering coal flow with Lower Heating Value (LHV) as a single input and electricity produced as a single output of the plant. The uncertainty of energy and exergy efficiency of the plant was evaluated with this method by applying sensitivity analysis depending on the effect of measurement parameters such as LHV, coal mass flow rate, cell generator output voltage/current. In addition, an extreme case analysis was investigated to determine the maximum range of the uncertainties. Results of the black box method showed that uncertainties varied between 1.82-1.98% for energy efficiency and 1.32-1.43% for exergy efficiency of the plant at an operating power level of 40-100% of full power. It was concluded that LHV determination was the most important uncertainty source of energy and exergy efficiency of the plant. The uncertainties of the extreme case analysis were determined between 2.30% and 2.36% for energy efficiency while 1.66% and 1.70% for exergy efficiency for 40-100% power output respectively. Proposed method was shown to be an approach for understanding major uncertainties as well as effects of some measurement parameters in a large scale thermal power plant. (C) 2014 Elsevier Ltd. All rights reserved.