Effects of Different Radiofrequency Fields on Oxidative Stress and Inflammatory Cytokine in the Stomach Tissue Under Indomethacin Application”

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Oruç S., Eraslan E., Göçmen A. Y., Özkut M. M., Aral B.

5th International 34th National Biophysics Congress, İzmir, Türkiye, 6 - 09 Eylül 2023, ss.152, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: İzmir
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.152
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Gazi Üniversitesi Adresli: Evet

Özet

Abstract

Aim: With the advancement of technology in recent years, the percentage of the general population exposed to radiofrequency field (RFF) has increased significantly. Also, increasing use of drugs leads to the destruction of the gastric and intestinal mucosa, and the deterioration of the balance between protective and destructive factors. The aim of this study is to examine the effects of 2100 MHz and 2600 MHz RFF exposure on gastric tissue, together with indomethacin (IND), one of the most commonly prescribed NSAID for patients.

Materials and Methods: 56 Wistar albino male rats (250-300g) were used with 7 groups; control, sham, IND, 2100 MHz, 2600 MHz, IND + 2100 MHz, and IND + 2600 MHz. The study was approved by Gazi University Animal Experiments Local Ethical Committee (G.Ü.ET-20.025). In the IND group, 4mg/kg indomethacin was administered for 15 days (5 days/week) by intragastric gavage. In RFF groups, rats were placed in plexiglass boxes for 15 days (5days/week) and exposed to RFF for 1 hour every day. In the IND+RFF groups, after 1 hour of exposure to RFF, 4 mg/kg indomethacin was administered by intragastric gavage for 15 days. At the end of 15 days, the animals were decapitated and stomach tissues were biochemically evaluated for cytokine and oxidative stress levels. HSP70 (Heat shock protein-70) and apoptotic cell count were performed in histological measurements. Each area was histologically scored on a 0-4 scale: 0-normal;1–epithelial cell damage; 2–glandular disruption; 3-mucosal disruption; 4-diffuse mucosal disruption. IBM SPSS Statistics program was used for analysis.

Results: RFF exposure combined with IND significantly increased oxidative stress and inflammation in gastric tissue compared to control, sham, and IND groups (p˂0.05). 2600 MHz application with emphasis on IND+RFF groups increased oxidative damage stronger than 2100 MHz (p˂0.05). Especially, in the IND+2600 MHz group, the number of HSP70 and apoptotic cells increased significantly (p˂0.05).

Conclusion:This study emphasizes that RFF exposure had a significant effect on gastric damage and triggered more damage to gastric tissue with IND application. It also emphasizes that patients using chronic NSAIDs should pay attention to the use of mobile phones more.

Keywords: Radiofrequency Field, Gastric Tissue, Indomethacin, OxidativeStress