Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats
JOURNAL OF ETHNOPHARMACOLOGY, cilt.112, sa.1, ss.145-151, 2007 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 112 Sayı: 1
- Basım Tarihi: 2007
- Doi Numarası: 10.1016/j.jep.2007.02.013
- Dergi Adı: JOURNAL OF ETHNOPHARMACOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.145-151
- Anahtar Kelimeler: Vitis vinifera, hepatoprotective, carbon tetrachloride, histopathology, MDA, GSH, AST, ALT, INDUCED HEPATOTOXICITY, SILYMARIN, MECHANISM, FRUITS
- Gazi Üniversitesi Adresli: Evet
Özet
The hepatoprotective effect of ethanolic extract and its four different fractions (CHCl3, EtOAc, n-BuOH, and remaining water fraction) of Vitis vinifera L. leaves was investigated against carbon tetrachloride (CCl4)-induced acute hepatotoxicity in rats. The ethanolic extract was found active at 125 mg/kg dose (per os). The ethanolic extract was fractionated through successive solvent-solvent extractions and the n-BuOH fraction in 83 mg/kg dose possessed remarkable antioxidant and hepatoprotective activities. Liver damage was assessed by using biochemical parameters (plasma and liver tissue MDA [malondialdehyde], transaminase enzyme levels in plasma [AST-aspartate transaminase, ALT-alanine transferase] and liver GSH [glutathione] levels). Additionally, the pathological changes in liver were evaluated by histopathological studies. Legalon 70 (R) Protect was used as standard natural originated drug. (C) 2007 Elsevier Ireland Ltd. All rights reserved.