Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats

Orhan D., Orhan N., Ergun E., Ergun F.

JOURNAL OF ETHNOPHARMACOLOGY, cilt.112, sa.1, ss.145-151, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 112 Sayı: 1
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.jep.2007.02.013
  • Dergi Adı: JOURNAL OF ETHNOPHARMACOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.145-151
  • Anahtar Kelimeler: Vitis vinifera, hepatoprotective, carbon tetrachloride, histopathology, MDA, GSH, AST, ALT, INDUCED HEPATOTOXICITY, SILYMARIN, MECHANISM, FRUITS
  • Gazi Üniversitesi Adresli: Evet

Özet

The hepatoprotective effect of ethanolic extract and its four different fractions (CHCl3, EtOAc, n-BuOH, and remaining water fraction) of Vitis vinifera L. leaves was investigated against carbon tetrachloride (CCl4)-induced acute hepatotoxicity in rats. The ethanolic extract was found active at 125 mg/kg dose (per os). The ethanolic extract was fractionated through successive solvent-solvent extractions and the n-BuOH fraction in 83 mg/kg dose possessed remarkable antioxidant and hepatoprotective activities. Liver damage was assessed by using biochemical parameters (plasma and liver tissue MDA [malondialdehyde], transaminase enzyme levels in plasma [AST-aspartate transaminase, ALT-alanine transferase] and liver GSH [glutathione] levels). Additionally, the pathological changes in liver were evaluated by histopathological studies. Legalon 70 (R) Protect was used as standard natural originated drug. (C) 2007 Elsevier Ireland Ltd. All rights reserved.