Effects of <i>Bacillus thuringiensis</i> <i>kurstaki</i> on Midgut Cells of <i>Thaumetopoea</i> <i>pityocampa</i> (Lepidoptera: Thaumetopoeidae), The Pine Processionary Caterpillar

UZUNHİSARCIKLI M., KALENDER Y., Suludere Z.

GAZI UNIVERSITY JOURNAL OF SCIENCE, cilt.27, sa.2, ss.861-870, 2014 (ESCI, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 2
  • Basım Tarihi: 2014
  • Dergi Adı: GAZI UNIVERSITY JOURNAL OF SCIENCE
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus
  • Sayfa Sayıları: ss.861-870
  • Gazi Üniversitesi Adresli: Evet

Özet

In this study effects of Bacillus thuringiensis kurstaki (Btk) on midgut cells of Thaumetopoea pityocampa larvae was investigated by electron microscopy. 3 mg/l Btk was given with food. 1 h, 3 h and 6 h after Btk administration no pathological changes in the midgut epitelial cells were observed. 12 h after Btk administration swelling of mitochondria in midgut epitelial cells were observed. Formation of vacuoles in cytoplasm was seen after 24 h. After 48 h decreasing of microvilli of midgut epithelial cell's and partial dissolving of cytoplasm, dissolving of nucleoplasm and chromatin clumps were observed. 60 h after Btk administration swelling and dissolving of mitochondria in midgut epithelial cells were detected. 96 h after Btk administration a membrane-body and dissolving in the cytoplasm clumps in midgut cells of T. pityocampa larvae were occurred. Present study shows that Btk is effective to T. pityocampa larvae.