Akademik Veri Yönetim Sistemi
4th International 33rd National Biophysics Congress 2022, Adıyaman, Türkiye, 31 Ağustos - 03 Eylül 2022, ss.79
Aim: It is mentioned in several reports that hyperthermic treatments may have a good impact on cancer healing. That's why successful applications of hyperthermia are increasing as a side treatment of cancer. This study, it was aimed to investigate the response of leukemia cells (MEC-1) and healthy cells (Human Fibroblast cells-hFB) to hyperthermia.
Material and Methods: After the Human Healthy Fibroblast (hFB) and leukemia (MEC-1) cells were cultured under appropriate conditions, they were exposed to 42°C and 43°C for different periods and then incubated for 24 hours. Then, cell proliferation (Cell Proliferation Kit, BI) tests with XTT, apoptosis and necrosis (eBioscience-Annexin V-FITC Apoptosis Detection Kit) and cell cycle analysis (Abcam Cell Cycle Assay Kit) tests were performed by flow cytometry. Student's t-test was used to evaluate the statistical results.
Results: After exposure of hFB cells at 42°C for 0, 5, 15, and 20 minutes, the total death rate was 6%, 25%, 27%, and 32%, respectively; Total death was detected in 5%, 20%, 27% and 78% of MEC-1 cells. At 43°C, after exposures of 0, 15, 30, and 45 minutes, total death was detected in hFB cells at the rates of 4%, 55%, 56%, 75%, respectively; Total death rates of MEC-1 cells were 7%, 28%, 34% and 58%.
Conclusion: The effectiveness of hyperthermia varies depending on temperature and exposure time. Our results reveal that heating at 42˚C and 43°C induced cell apoptosis and necrosis, and inhibited cell proliferation. However, our data showed that exposure to hyperthermia at 42°C drives cells towards apoptosis, but at higher temperatures, cells prefer to undergo necrosis rather than apoptosis in healthy and cancer cells.