Fabrication of SERS active gold nanorods using benzalkonium chloride, and their application to an immunoassay for potato virus X

ÇAĞLAYAN M. G., Kasap E., ÇETİN D., Suludere Z., TAMER U.

MICROCHIMICA ACTA, cilt.184, sa.4, ss.1059-1067, 2017 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 184 Sayı: 4
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1007/s00604-017-2102-x
  • Dergi Adı: MICROCHIMICA ACTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1059-1067
  • Anahtar Kelimeler: Nanoparticles, Surfactant, Particle capping, Raman spectroscopy, Surface enhanced raman spectroscopy, Sandwich immunoassay, Magnetic nanoextraction, CETYLTRIMETHYLAMMONIUM BROMIDE, NANOPARTICLE, SURFACE, GROWTH, SUBSTRATE, SHAPE, SCATTERING, NANOCUBES, AU
  • Gazi Üniversitesi Adresli: Evet

Özet

The authors report on a robust method for the synthesis of gold nanorods (AuNRs) with tunable dimensions and longitudinal surface plasmon resonance. The method relies on seed-mediated particle growth in the presence of benzalkonium chloride (BAC) in place of the widely used surfactant cetyltrimethyl ammonium bromide (CTAB). Uniform AuNRs were obtained by particle growth in solution, and BAC is found to stabilize the AuNRs for > 1 year. The SERS activity of the resulting AuNRs is essentially identical to that of CTAB-protected nanorods. The SERS activity of the BAC protected nanorods was applied to the quantitative analysis of potato virus X (PVX). The calibration plot for PVX is linear in the 10 to 750 ngai...mL(-1) concentration range, and the detection limit is 2.2 ngai...mL(-1).