Significant association of interleukin-4 gene intron 3 VNTR polymorphism with susceptibility to knee osteoarthritis

YİĞİT S., Inanir A., Tekcan A., Tural E., Ozturk G. T., KISMALI G., ...Daha Fazla

Gene, cilt.537, sa.1, ss.6-9, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 537 Sayı: 1
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.gene.2013.12.060
  • Dergi Adı: Gene
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.6-9
  • Anahtar Kelimeler: Interleukin-4 (IL-4), Knee osteoarthritis, Polymorphism
  • Gazi Üniversitesi Adresli: Hayır

Özet

Objective: Interleukin-4 (IL-4) is a strong chondroprotective cytokine and polymorphisms within this gene may be a risk factor for osteoarthritis (OA). We aimed to investigate genotype and allele frequencies of IL- 4 gene intron 3 variable number of tandem repeats (VNTR) polymorphism in patients with knee OA in a Turkish population. Methods: The study included 202 patients with knee OA and 180 healthy controls. Genomic DNA was isolated and IL- 4 gene 70. bp VNTR polymorphism determined by using polymerase chain reaction (PCR) with specific primers followed by restriction fragment length polymorphism (RFLP) analysis. Results: Our result show that there was statistically significant difference between knee OA patients and control group with respect to IL- 4 genotype distribution and allele frequencies (p. = 0.000, OR: 0.20, 95% CI: 0.10-0.41, OR: 0.22, 95% CI: 0.12-0.42, respectively). Conclusions: Our findings suggest that there is an association of IL- 4 gene intron 3 VNTR polymorphism with susceptibility of a person for development of knee OA. As a result, IL- 4 gene intron 3 VNTR polymorphism could be a genetic marker in OA in a Turkish study population. This is the first association study that evaluates the associations between IL-4 gene VNTR polymorphism and knee OA. © 2013.