The Impact of As-Built Surface Characteristics of Selective-Laser-Melted Ti-6Al-4V on Early Osteoblastic Response for Potential Dental Applications


Razzaq M. H., Ayeni O., Köklü S., Berk K., Zaheer M. U., Tjardts T., ...More

Journal of Functional Biomaterials, vol.16, no.7, 2025 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 16 Issue: 7
  • Publication Date: 2025
  • Doi Number: 10.3390/jfb16070230
  • Journal Name: Journal of Functional Biomaterials
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Applied Science & Technology Source, BIOSIS, Communication Abstracts, INSPEC, Metadex, Directory of Open Access Journals, Civil Engineering Abstracts
  • Keywords: dental implant, osteoblast, Selective Laser Melting (SLM), surface topography, TiAl6V4
  • Gazi University Affiliated: Yes

Abstract

This study investigates the potential of Selective Laser Melting (SLM) to tailor the surface characteristics of Ti6Al4V directly during fabrication, eliminating the need for post-processing treatments potentially for dental implants. By adjusting the Volumetric Energy Density (VED) through controlled variations in the laser scanning speed, we achieved customized surface textures at both the micro- and nanoscale levels. SLM samples fabricated at moderate VED levels (50–100 W·mm3/s) exhibited optimized dual-scale surface roughness—a macro-roughness of up to 25.5–27.6 µm and micro-roughness of as low as 58.8–64.2 nm—resulting in significantly enhanced hydrophilicity, with water contact angles (WCAs) decreasing to ~62°, compared to ~80° on a standard grade 5 machined Ti6Al4V plate. The XPS analysis revealed that the surface oxygen content remains relatively stable at low VED values, with no significant increase. The surface topography plays a significant role in influencing the WCA, particularly when the VED values are low (below 200 W·mm3/s) during SLM, indicating the dominant effect of surface morphology over chemistry in these conditions. Biological assays using osteoblast-like MG-63 cells demonstrated that these as-built SLM surfaces supported a 1.5-fold-higher proliferation and improved cytoskeletal organization relative to the control, confirming the enhanced early cellular responses. These results highlight the capability of SLM to engineer bioactive implant surfaces through process-controlled morphology and chemistry, presenting a promising strategy for the next generation of dental implants suitable for immediate placement and osseointegration.