Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some Turkish medicinal plants

Orhan İ., Sener B., Choudhary M., Khalid A.

Journal of Ethnopharmacology, vol.91, no.1, pp.57-60, 2004 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 91 Issue: 1
  • Publication Date: 2004
  • Doi Number: 10.1016/j.jep.2003.11.016
  • Journal Name: Journal of Ethnopharmacology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.57-60
  • Keywords: anticholinesterase activity, acetylcholinesterase, butyrylcholinesterase, Alzheimer's disease, Ellman method, ALZHEIMERS-DISEASE, SENILE DEMENTIA, PREVALENCE
  • Gazi University Affiliated: Yes


The chloroform:medianol (1:1) extracts of a number of the plant species belonging to eight families, namely Corydalis solida (L.) Swartz subsp. solida and Glaucium corniculatum (L.) J. H. Rudolph (Papaveraceae), Rhododendron ponticum L. subsp. ponticum and Rhododendron luteum Sweet. (Ericaceae), Buxus sempervirens L. (Buxaceae), Vicia faba L. (Fabaceae), Robinia pseudoacacia L. (Caeselpiniaceae), Tribulus terrestris L. and Zygophyllum fabago L. (Zygophyllaceae), Lycopodium clavatum L. (Lycopodiaceae), Fumaria vaillantii Lois., Fumaria capreolata L., Fumaria kralikii Jordan, Fumaria asepala Boiss., Fumaria densiflora DC., Fumaria flabellata L., Fumaria petteri Reichb. subsp. thuretii (Boiss.) Pugsley, Fumaria macrocarpa Boiss. ex Hausskn., Fumaria cilicica Hauskkn., Fumaria parviflora Lam. and Fumaria judaica Boiss. (Fumariaceae) were screened for their anticholinesterase activity on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes by in vitro Ellman method at 10μg/ml and 1mg/ml concentrations. The extracts did not show any noticeable inhibitory activity against both of the enzymes at 10μg/ml. The extracts of Rhododendron ponticum subsp. ponticum, Rhododendron luteum, Corydalis solida subsp. solida, Glaucium corniculatum, and Buxus sempervirens showed remarkable inhibitory activity above 50% inhibition rate on AChE at 1mg/ml. Among them, Rhododendron ponticum subsp. ponticum, Corydalis solida subsp. solida and Buxus sempervirens were the most active extracts against BChE having 95.46±1.03%, 93.08±0.97%, and 93.45±0.88% inhibition rates, respectively. Among the extracts screened, all of the Fumaria extracts displayed highly potent inhibition against both of the enzymes at 1mg/ml concentration compared to the standard. © 2003 Elsevier Ireland Ltd. All rights reserved.