Baculoviral vector loaded mesenchymal stem cells as efficient gene therapy tools for cancer treatment


Aktas S. H. , AKBULUT H., ELÇİN A. E. , PARMAKSIZ M., Keskin A. A. , Coleri Cihan A., ...Daha Fazla

TURKISH JOURNAL OF BIOLOGY, cilt.40, sa.5, ss.1121-1128, 2016 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 40 Konu: 5
  • Basım Tarihi: 2016
  • Doi Numarası: 10.3906/biy-1601-76
  • Dergi Adı: TURKISH JOURNAL OF BIOLOGY
  • Sayfa Sayıları: ss.1121-1128

Özet

Insufficient targeting of the therapeutic genes to tumor cells is one of the major reasons for failure in cancer gene therapy. Mesenchymal stem cells (MSCs) seem to be a good candidate as a carrier for gene therapy because of its selective tumor tissue-homing properties. In the current study, we constructed baculoviral vectors (BVs) carrying cytosine deaminase (CD) (BV-CD) or green fluorescence protein (GFP) genes (BV-GFP) and tested the transduction efficiency of the vectors in tumor and mesenchymal stem cells. We also tested the in vivo efficacy of the BV-CD vector in a colon cancer model. Our results showed that the recombinant baculoviral vectors can efficiently transduce mammalian cells and express genes of interest. The BV-CD vector treatment caused significant in vitro cytotoxicity when used with 5-fluorocytosine. MSCs loaded with the BV-CD vector caused a significant delay in tumor growth and increased survival when compared to control and MSC alone treated groups bearing colon cancer.