Effects of Lyophilized Leech Saliva Extract on in vitro Cell migration and Apoptotic/ Necrotic Cell Death on the Human Breast Cancer Cell Line (MDA-MB-231)

Creative Commons License

Ünal K.

International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina – ABMBBIH Conference 2023,, Sarajevo, Bosna-Hersek, 18 - 20 Mayıs 2023, cilt.7, sa.2

  • Yayın Türü: Bildiri / Yayınlanmadı
  • Cilt numarası: 7
  • Basıldığı Şehir: Sarajevo
  • Basıldığı Ülke: Bosna-Hersek
  • Gazi Üniversitesi Adresli: Evet

Özet

Medical leech therapy/ Hirudotherapy, which is in high demand in many countries, especially in Turkiye, China, India and Iran, is a traditional and complementary treatment method with a centuries-old history. Medical leech therapy contributes to the treatment of diseases by showing anti-inflammatory, antimicrobial, analgesic, vasodilator and/or anesthetic effects. The benefits obtained from the leech therapy are mainly due to the various bioactive substances found in their saliva, which secreted into the host's blood stream during blood sucking. In this study, the effects of lyophilized leech saliva extract on the cell viability, cell migration, apoptosis, necrosis and possible mRNA expression responsible for these effects were evaluated under in vitro conditions. Leech saliva was obtained from sterile medicinal leeches of Hirudo verbana genus and then this saliva was lyophilized. The total protein concentration from this saliva was determined and then the dose amounts (800 µg/ml, 400 µg/ml, 200 µg/ml and 100 µg/ml) were adjusted by serial dilution. In this study, the Human Breast Cancer cell line (MDA-MB-231) and Human Umbilical Cord Vein Endothelial Cell (HUVEC-CRL-1730) line were used. An MTT assay, cell migration assay, PCR and flow cytometry analysis were performed. The results indicated that lyophilized leech saliva extract can reduce cell viability, cell migration and induce necrosis along with apoptosis in this cancer cell line. In this study, it was found that the lyophilized leech saliva extract applied at different doses had significant effects on the MDA-MB-231. This study investigated for the first time that lyophilized leech saliva extract has the potential effect in the MDA-MB-231. LLSE applied at different doses had significant and different effects on the MDA-MB-231 cancerous-cell line and HUVEC healthy-cell line. The results indicated that LLSE dose-dependently decreases cell proliferation and migration in the MDA-MB-231 cell line.