Glucose biosensor based on immobilization of glucose oxidase on a carbon paste electrode modified with microsphere-attached l-glycine


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Donmez S., Arslan F. , Sarı N. , Hasanoğlu Özkan E. , Arslan H.

Biotechnology and Applied Biochemistry, cilt.64, ss.745-753, 2017 (SCI Expanded İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 64
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1002/bab.1533
  • Dergi Adı: Biotechnology and Applied Biochemistry
  • Sayfa Sayıları: ss.745-753

Özet

In the present study, a novel biosensor that is sensitive to glucose was prepared using the microspheres modified with (4-formyl-3-methoxyphenoxymethyl)polystyrene (FMPS) with L-glycine. Polymeric microspheres having Schiff bases were prepared from FMPS using the glycine condensation method. Glucose oxidase enzyme was immobilized onto modified carbon paste electrode by cross-linking with glutaraldehyde. Oxidation of enzymatically produced H2O2 (+0.5 V vs. Ag/AgCl) was used for determination of glucose. Optimal temperature and pH were found as 50 ◦C and 8.0, respectively. The glucose biosensor showed a linear working range from 5.0 × 10−4 to 1.0 × 10−2 M, R2 = 0.999. Storage and operational stability of the biosensor were also investigated. The biosensor gave perfect reproducible results after 20 measurements with 3.3% relative standard deviation. It also had good storage stability.