Novel dendrimers containing redox mediator: Enzyme immobilization and applications

YETİM N. K., SARI N.

JOURNAL OF MOLECULAR STRUCTURE, cilt.1191, ss.158-164, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1191
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.molstruc.2019.04.090
  • Dergi Adı: JOURNAL OF MOLECULAR STRUCTURE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.158-164
  • Anahtar Kelimeler: Dendrimer, Ferrocene, Immobilization, Glucose oxidase, Diabetes mellitus, GLUCOSE-OXIDASE, FERROCENE, COVALENT, MACROMOLECULES, MICROSPHERES
  • Gazi Üniversitesi Adresli: Evet

Özet

In this study, new dendrimers with a ferrocene core were produced by means of a divergent method for the immobilization of the glucose oxidase enzyme (GOx). Pt(II) and Pt(IV) ions were attached to the terminal groups of dendrimers. Metal-containing dendrimers, [Fc(MGlu)(2)-Aph-Pt(II)] and [Fc(MGlu)(2)-Aph-Pt(IV)], were obtained using the "template method" with aminophenol and Pt(II)/Pt(IV) cations. These compounds have been characterized by molar conductivity, magnetic susceptibility, FTIR, UV-Vis, H-1 NMR and LC-MS methods. The GOx enzyme was immobilized on the Fc(MGlu)(2), [Fc(MGlu)(2)-Aph-Pt(II)] and [Fc(MGlu)(2)-Aph-Pt(IV)] dendrimers, and the immobilized enzyme optimization parameters (substrate concentration, temperature, pH, reusability and storage capacity) were determined. Their K-m (mM) and V-max (mM.min(-1)) values were calculated from the Michaelis-Menten equation. The reusability of the immobilized glucose oxidase enzyme was investigated in an artificial urine medium. The research showed that [Fc(MGlu)(2)-Aph-Pt(II)] retains more than 61.23% of its initial activity after 10 successive cycles, which is a remarkable result. (C) 2019 Elsevier B.V. All rights reserved.