Relationships between Interleukin 18-607 C/A and-137 G/C, Osteopontin-9250 C/T Genetic Polymorphisms and Systemic Inflammatory Response Syndrome in Coronary Artery Bypass Graft Surgery

KÖSE S. K., Karahilal B., Engin B., Aydoğdu G., Yağar S., ORHAN K.

MEDICINA-LITHUANIA, cilt.60, sa.5, 2024 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 60 Sayı: 5
  • Basım Tarihi: 2024
  • Doi Numarası: 10.3390/medicina60050724
  • Dergi Adı: MEDICINA-LITHUANIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Directory of Open Access Journals
  • Anahtar Kelimeler: CABG surgery, interleukin 18 -137 G/C genetic polymorphism, interleukin 18 -607 C/A genetic polymorphism, osteopontin 9250 C/T genetic polymorphism, systemic inflammatory response syndrome
  • Gazi Üniversitesi Adresli: Evet

Özet

Background and Objectives: Systemic inflammatory response syndrome (SIRS) is one of the most significant complications after on-pump heart surgery procedures. High cytokine levels have been shown after open-heart surgeries and a genetic predisposition seems to be an important underlying modulatory characteristic for SIRS. To investigate the association between interleukin 18 -607 C/A, interleukin 18 -137 G/C and osteopontin 9250 C/T genetic polymorphisms and SIRS in on-pump CABG patients. Materials and Methods: Two hundred consecutive elective on-pump CABG patients were recruited prospectively to the study. Genomic DNA was extracted from whole blood and genotyping was determined by sequence specific PCR or PCR-RFLP methods for related polymorphisms. Results: SIRS incidence was 60.2%, 38.1%, 18.9% on postoperative day 1, 2 and 3, respectively, in the whole study population. The SIRS rate on the second postoperative day was 13% and 43.4%, respectively, in osteopontin 9250 C/T T allele non-carriers and carriers (p = 0.004). WBC (White Blood Cell) counts were higher on day 2 and 3 in osteopontin 9250 C/T T allele carriers compared to non-carriers (day 2; 12.7 +/- 4 vs. 10.5 +/- 2.4 (p = 0.015), day 3; 11.8 +/- 4 vs. 9.1 +/- 4.7 (p = 0.035)). The average ICU stay was 3.1 +/- 7.4, 1.28 +/- 0.97 for IL 18-137 G/C C allele carriers and non-carriers, respectively (p = 0.003), and in the IL 18-137 G/C C allele carriers, SIRS developed in 42.2% by the second postoperative day whereas the rate was 57.8% in non-carriers (p = 0.025). Conclusions: The current research revealed a possible link between osteopontin 9250 C/T and IL18-137 G/C genetic polymorphism and SIRS and morbidity in on-pump CABG patients.