Akademik Veri Yönetim Sistemi
4. INTERNATIONAL SELÇUK SCIENTIFIC RESEARCH AND INNOVATION CONGRESS, Konya, Türkiye, 24 - 25 Ağustos 2024, ss.121-122, (Özet Bildiri)
Cancer is the second leading cause of death worldwide after cardiovascular disease.
Pancreatic cancer (PC), the fourth most common cause of cancer deaths, is an aggressive form
of cancer with a five-year survival rate of less than 5% and an average survival of 4-6 months.
Due to the sudden development of PC after diagnosis and the lack of specific symptoms, the
success of appropriate treatment is very limited. Treatment alternatives for PC include
chemotherapy, radiotherapy and surgery. In recent years, with the development of technology,
new methods have been used in addition to cancer treatments. Different therapeutic agents
show promising efficacy against various cancers. The use of medicinal leeches has become a
popular method in traditional medicine due to the various bioactive components found in
leech secretion. In our study, the cytotoxic and genotoxic effects of the secretion extract
obtained from the medicinal leech Hirudo verbana on MIA PaCa-2 (human pancreatic cancer
cells) cell lines were demonstrated using XTT method. Leech secretion was obtained from
medical leeches of Hirudo verbana species and kept at -80°C for 24 hours. Then, the secretion
obtained was placed in the lyophiliser for 48 hours and brought into solid powder form. Total
protein concentration of the obtained powder secretion form was determined and dose
amounts were adjusted by serial dilution. XTT test was performed to determine cell viability
and LC50 value at 24-48-72 hours was calculated according to nonlinear regression. IC50
doses were determined as 447.76 μM at 24 hours, 332.40 μM at 48 hours and 538.11 μM at 72
hours, respectively. In the cancer cell line (MIA PaCa-2) to which different doses of leech
secretion were applied, the effect on cell viability varied depending on time and it was
determined that the effect increased at high concentration. It shows that medical leech
secretion has cytotoxic and genotoxic effects in MIA PaCa-2 (human pancreatic cancer cells).