In vitro bioactivity, cytocompatibility and amoxicillin delivery of APTES-modified cerium- and magnesium-loaded calcium silicate biomaterials
MATERIALS TODAY CHEMISTRY, cilt.56, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 56
- Basım Tarihi: 2026
- Doi Numarası: 10.1016/j.mtchem.2026.103996
- Dergi Adı: MATERIALS TODAY CHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Compendex, INSPEC
- Anahtar Kelimeler: Amoxicillin release, APTES, Bone tissue regeneration, Calcium silicate, Cerium/magnesium, Eggshell, In vitro bioactivity
- Gazi Üniversitesi Adresli: Evet
Özet
Mesoporous calcium silicate biomaterials were synthesized using eggshell-derived calcium and modified with 3-aminopropyl triethoxysilane (APTES), cerium (Ce), and magnesium-cerium (MgCe). The materials were characterized by N(2)adsorption/desorption, XRD, FTIR, SEM/EDS and ICP-OES. BET surface areas ranged from 485 to 1036 m(2)/g. In-vitro bioactivity was confirmed via hydroxyapatite formation in SBF after 28 days. Cytotoxicity studies on HaCaT, HUVEC, and CCD-18Co cell lines demonstrated high biocompatibility (IC50 > 140 mu g/mL for all formulations). Addition osteoblast viability on hBM-MSCs stem cells maintained high IC(50)thresholds exceeding 770 mu g/mL. Amoxicillin loading capacities of 110-138 mg/g were achieved. CSB@APTES showed the highest loading capacity (137.83 mg/g) and loading efficiency (51.80%), followed by CSB@APTES-Ce and CSB@APTES-MgCe. After 72 h in SBF, drug release reached 37.6%, 24.8%, and 20.7%, respectively. Controlled release in SBF (pH 7.4) over 72 h followed anomalous (non-Fickian) transport, best described by the Peppas-Sahlin model. Ce and MgCe doping reduced release rate, enabling tunable drug delivery. These biomaterials show promise for dual-function bone tissue engineering applications.