Neuroprotective effects of topical coenzyme Q10+vitamin E in mechanic optic nerve injury model


Ekicier Acar S., Saricaoglu M. S. , Colak A., AKTAŞ Z. , SEPİCİ DİNÇEL A.

EUROPEAN JOURNAL OF OPHTHALMOLOGY, vol.30, no.4, pp.714-722, 2020 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 4
  • Publication Date: 2020
  • Doi Number: 10.1177/1120672119833271
  • Title of Journal : EUROPEAN JOURNAL OF OPHTHALMOLOGY
  • Page Numbers: pp.714-722
  • Keywords: Rat glaucoma model, neuroprotection, coenzyme Q10+vitamin E, retinal ganglion cell number, MITOCHONDRIAL TRANSCRIPTION FACTOR, PROTECTS RETINAL CELLS, OXIDATIVE STRESS, ALPHA-TOCOPHEROL, DBA/2J MOUSE, IN-VITRO, GLAUCOMA, DAMAGE, DEATH, OVEREXPRESSION

Abstract

Purpose: We aimed to create mechanic optic nerve injury model in rats and investigate the neuroprotective effects of topical Coenzyme Q10 + Vitamin E (CoQ + Vit.E) molecules on retinal ganglion cells. Methods: Mechanic optic nerve injury model was created in the right eyes of rats (n = 12). Rats were divided into two groups: glaucoma model with sham treatment and topical CoQ + Vit.E treatment. Treatment was applied for 4 weeks. Glial fibrillary acidic protein, Brn-3a antibody, and anti-Iba1 were examined by immunohistochemistry. Glial fibrillary acidic protein, Bax, Bcl-xL, and Tfam protein expression were measured by Western blot analysis. Results: The number of Brn-3a-positive retinal ganglion cell was 15.0 +/- 1.0 (min: 14, max: 16) in sham treatment group and 22.2 +/- 4.8 (min: 18, max: 29) in topical CoQ10 + Vit.E treatment group. The protection of Brn-3a in CoQ10 + Vit.E was statistically significant (p < 0.05). Glial fibrillary acidic protein-positive astroglial counts were recorded as 11.7 +/- 2.1 (min: 10, max: 14) in sham treatment and 2.5 +/- 1.5 (min: 1, max: 4) in topical CoQ10 + Vit.E treatment group (p < 0.05). Topical CoQ10 + Vit.E treatment also decreased Iba1 expression in the retina of mechanic optic nerve injury groups. CoQ10 + Vit.E treatment prevented apoptotic cell death by increasing Bcl-xL protein expression. Also, CoQ10 + Vit.E preserved Tfam protein expression in the retina. Conclusion: This study has shown that in glaucoma treatment the neuron protecting effect of topical CoQ10 + Vit.E molecules can be valuable.