EPITHELIUM-DEPENDENT RESPONSES OF SEROTONIN IN A COAXIAL BIOASSAY SYSTEM


CAKICI I., TUNCTAN B., ABACIOGLU N., KANZIK I.

EUROPEAN JOURNAL OF PHARMACOLOGY, vol.236, no.1, pp.97-105, 1993 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 236 Issue: 1
  • Publication Date: 1993
  • Doi Number: 10.1016/0014-2999(93)90231-6
  • Title of Journal : EUROPEAN JOURNAL OF PHARMACOLOGY
  • Page Numbers: pp.97-105

Abstract

Serotonin (10(-6)-10(-4) M) produced relaxations in a concentration-dependent (at 10(-6) and 10(-5) M concentrations) manner followed by a contraction (at 10(-4) M concentration) in a co-axial system, which consisted of guinea-pig trachea as a donor organ for epithelial derived-relaxing factor(s) and phenylephrine-precontracted rat anococcygeus muscle as assay tissue. Serotonin produced a concentration-dependent contraction only in precontracted rat anococcygeus muscle mounted alone or mounted co-axially within epithelium-denuded trachea. Indomethacin (10(-6) M) significantly inhibited the initial relaxations (from 25.1 +/- 7.8 to 7.8 +/- 5.0% and from 35.6 +/- 8.7 to 10.4 +/- 8.3% at 10(-6) and 10(-5) M concentrations of serotonin), but did not affect the contraction. Imipramine (10(-8) M) and hydrocortisone (3 X 10(-5) M) reduced the initial relaxations (from 20.5 +/- 1.6 to 3.8 +/- 1.5% and from 32.1 +/- 6.4 to 18.9 +/- 3.9% at 10(-6) M and 10(-5) M concentrations of serotonin, respectively) and also converted the serotonin (10(-4) M)-induced contraction to a relaxation. In the co-axial system with trachea from guinea-pigs previously sensitized with i.p. injected egg-ovalbumin, the serotonin-induced biphasic response was converted to a contractile response only after ovalbumin challenge. Histopathologic changes were observed in the epithelium of challenged tracheas taken from sensitized guinea-pigs and alterations of serotonin-induced epithelium-dependent responses were attributed to the morphological and/or functional damage of tracheal epithelium caused by ovalbumin challenge. In the modified co-axial system, phenylephrine-induced contractions faded quickly when the rat anococcygeus muscle was mounted in epithelium-intact guinea-pig trachea, and the percentage fade was significantly higher (92.3 +/- 2.8%) than that obtained when the anococcygeus muscle was mounted in epithelium-denuded trachea (56.9 +/- 8.4%) or when it was mounted alone (44.6 +/- 7.7%). Our results suggest that guinea-pig tracheal epithelium is capable of modulating the responsiveness of rat anococcygeus muscle to serotonin by affecting the basal or stimulated release of some inhibitory mediators.