Biochemical analysis of Centaurea depressa phenylalanine ammonia lyase (PAL) for biotechnological applications in phenylketonuria (PKU)

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Babaoğlu Aydaş S. S., Sirin S., Aslım B.

PHARMACEUTICAL BIOLOGY, cilt.54, sa.12, ss.2838-2844, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 54 Sayı: 12
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1080/13880209.2016.1185634
  • Dergi Adı: PHARMACEUTICAL BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2838-2844
  • Anahtar Kelimeler: Enzyme optimization, HPLC, PAL-specific activity, partial enzyme purification, western blotting, CELL-SUSPENSION CULTURES, MOLECULAR CHARACTERIZATION, PURIFICATION, ENZYME, GENES, THERAPY, PLANTS
  • Gazi Üniversitesi Adresli: Evet

Özet

Context: Phenylketonuria (PKU) is the most common hereditary defect of phenylalanine hydroxylase (PAH) enzyme achieving the hydroxylation of phenylalanine (Phe). Phenylalanine ammonia lyase (PAL) converts Phe to a harmless metabolite, trans-cinnamic acid (TCA) in plants and PAL enzyme activity is fairly high in plants rich in flavonoids.