Akademik Veri Yönetim Sistemi
2ndInternational Congress of Multidisciplinary Medical and Health Sciences Studies (ICOMMEH 2024), Ankara, Türkiye, 27 - 28 Mayıs 2024, ss.97
Reishi (Ganoderma lucidum (Curtis) P. Karst.) has long been known to exhibit a broad spectrum of biological effects. Ganoderma triterpenoids are known to have potential against Alzheimer's Disease by alleviating neuroinflammation and reducing neuronal apoptosis. There is no current study showing the cytotoxic effect of this fungus, which is widely used in Asian countries and our country, in studies conducted with cells. Therefore, in our study, the effects of different doses of Reishi extract on the stimulated SH5Y cell line were examined. For this purpose, Reishi extracts prepared at 5 different concentrations (500–31.25 µg/mL) were applied to the cells and incubated for 24 and 48 hours. At the end of each incubation, MTT levels for cytotoxicity, Annexin-V for apoptosis, and ROS levels for oxidative stress were measured. According to MTT results, it was observed that the highest dose, 500 µg/mL, showed cytotoxic activity in 48 hours, while other doses caused proliferation after 24 and 48 hours of incubation. It can be thought that reishi, an adaptogenic mushroom, shows this effect in low doses and increases proliferation. According to our study results, it was observed that ROS levels decreased compared to control, and apoptosis results were correlated with cytotoxicity results. It has been observed that this fungus, which is used for therapeutic and protective purposes, has a reducing reactive oxygen species and proliferation effect on cells. Dose selection is important and low doses may cause the proliferation of cells that are intended to be directed to apoptosis, thus negatively affecting the treatment.
Keywords: Reishi, apoptosis, cytotoxicity, ROS