Estimation of Cholinesterase Inhibitory and Antioxidant Effects of the Leaf Extracts of Anatolian Ficus carica var. domestica and their Total Phenol and Flavonoid Contents


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ERDOĞAN ORHAN İ. , ÜSTÜN O. , Sener B.

NATURAL PRODUCT COMMUNICATIONS, cilt.6, ss.375-378, 2011 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 6 Konu: 3
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1177/1934578x1100600315
  • Dergi Adı: NATURAL PRODUCT COMMUNICATIONS
  • Sayfa Sayıları: ss.375-378

Özet

Ficus carica var. domestica Tsch. & Rav. (common fig) is widely grown in Turkey and exported for its edible fruits. In this study, the n-hexane, chloroform, acetone, methanol, n-butanol, and water extracts of the leaves of F. carica var. domestica were screened for their cholinesterase inhibitory and antioxidant activities. Cholinesterase inhibition against acetyl- (AChE) and butyrylcholinesterase (BChE) was measured by the spectrophotometric method of Ellman at concentrations of 25, 50, and 100 mu g/mL., while antioxidant activity was tested using three in vitro methods; 2,2-diphenyl-l-picrylhydrazyl (DPPH) radical scavenging activity, metal-chelation capacity, and ferric-reducing antioxidant power (FRAP). Total phenol and flavonoid contents of the extracts were determined spectrophotometrically. Our results revealed that the n-hexane and acetone extracts exerted a notable inhibition against both AChE (62.9 +/- 0.9% and 50.8 +/- 2.1%, respectively) and BChE (76.9 +/- 2.2% and 45.6 +/- 1.3%, respectively). However, they had low activity in the antioxidant tests. The chloroform extract was found to be the richest in total flavonoid content (252.5 +/- 1.1 mg/g quercetin equivalent), while the n-butanol extract had the highest total phenol amount (85.9 +/- 3.2 mg/g extract gallic acid equivalent).