In this study spasticity of shoulder rotator muscle groups was evaluated with clinical and isokinetic methods and its relationship with hemiplegic shoulder pain was investigated. Thirty-one stroke patients and 15 healthy subjects were included in the study. Spasticity of shoulder internal and external rotator muscles were evaluated by using Ashworth scale and isokinetic dynamometer. Maximum torque (MT), work, ratios of internal and external rotators' MT and work were calculated. Work, MT and work ratios during external rotation were significantly different between patient and control groups. There was a significant correlation between Ashworth scores and MT, work values of shoulder internal rotators. Shoulder pain was found in 18 patients and glenohumeral subluxation in 25. The relationship between shoulder pain and subluxation was nonsignificant (c 2=0.199, p=0.676). Mean Ashworth scores, mean MT and work values of shoulder internal and external rotators were not different between patients with and without shoulder pain. Maximum torque and work ratios of patients with shoulder pain were higher in all angular velocities and the difference between groups was significant in 120°/sec angular velocity. As a result; we thought that imbalance between rotator muscle groups was important in hemiplegic shoulder pain.