Mutagenic and morphologic impacts of 1.8 GHz radiofrequency radiation on human peripheral blood lymphocytes (hPBLs) and possible protective role of pre-treatment with Ginkgo biloba (EGb 761)

EŞMEKAYA M. A., Aytekin E., ÖZGÜR BÜYÜKATALAY E., GÜLER ÖZTÜRK G., ERGÜN M. A., ÖMEROĞLU S., ...Daha Fazla

SCIENCE OF THE TOTAL ENVIRONMENT, cilt.410, ss.59-64, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 410
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.scitotenv.2011.09.036
  • Dergi Adı: SCIENCE OF THE TOTAL ENVIRONMENT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.59-64
  • Anahtar Kelimeler: Radiofrequency radiation, Sister chromatid exchange, Electron microscopy, Cell viability, Mutagenicity, SISTER-CHROMATID EXCHANGE, ENVIRONMENTAL NONIONIZING RADIATION, FREQUENCY ELECTROMAGNETIC-FIELDS, MOBILE PHONE RADIATION, GENE-EXPRESSION, CELL-PROLIFERATION, OXIDATIVE STRESS, DNA BREAKS, RAT-BRAIN, MICROWAVES
  • Gazi Üniversitesi Adresli: Evet

Özet

The mutagenic and morphologic effects of 1.8 GHz Global System for Mobile Communications (GSM) modulated RF (radiofrequency) radiation alone and in combination with Ginkgo biloba (EGb 761) pre-treatment in human peripheral blood lymphocytes (hPBLs) were investigated in this study using Sister Chromatid Exchange (SCE) and electron microscopy. Cell viability was assessed with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) reduction assay. The lymphocyte cultures were exposed to GSM modulated RF radiation at 1.8 GHz for 6,8, 24 and 48 h with and without EGb 761. We observed morphological changes in pulse-modulated RF radiated lymphocytes. Longer exposure periods led to destruction of organelle and nucleus structures. Chromatin change and the loss of mitochondrial crista occurred in cells exposed to RF for 8 h and 24 h and were more pronounced in cells exposed for 48 h. Cytoplasmic lysis and destruction of membrane integrity of cells and nuclei were also seen in 48 h RF exposed cells. There was a significant increase (p<0.05) in SCE frequency in RF exposed lymphocytes compared to sham controls. EGb 761 pre-treatment significantly decreased SCE from RF radiation. RF radiation also inhibited cell viability in a time dependent manner. The inhibitory effects of RF radiation on the growth of lymphoctes were marked in longer exposure periods. EGb 761 pre-treatment significantly increased cell viability in RF + EGb 761 treated groups at 8 and 24 h when compared to RF exposed groups alone. The results of our study showed that RF radiation affects cell morphology, increases SCE and inhibits cell proliferation. However, EGb 761 has a protective role against RF induced mutagenity. We concluded that RF radiation induces chromosomal damage in hPBLs but this damage may be reduced by EGb 761 pre-treatment (C) 2011 Elsevier B.V. All rights reserved.