Participation of the components of L-arginine/nitric oxide/cGMP cascade by chemically-induced abdominal constriction in the mouse


Abacioglu N., Tunctan B., Akbulut E., Cakici I.

LIFE SCIENCES, vol.67, no.10, pp.1127-1137, 2000 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 67 Issue: 10
  • Publication Date: 2000
  • Doi Number: 10.1016/s0024-3205(00)00711-6
  • Journal Name: LIFE SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1127-1137
  • Keywords: nitric oxide, L-arginine/NO/cCMP pathway, antinociception, abdominal constriction, mice, CYCLIC-GMP PATHWAY, ENDOGENOUS NITRIC-OXIDE, SPINAL-CORD, SYNTHASE INHIBITORS, ANTINOCICEPTIVE ACTIVITY, THERMAL HYPERALGESIA, POSSIBLE INVOLVEMENT, GUANYLATE-CYCLASE, NADPH-DIAPHORASE, 7-NITRO INDAZOLE
  • Gazi University Affiliated: No

Abstract

The purpose of this study was to investigate the role of the L-arginine/nitric oxide (NO)/cGMP pathway in p-benzoquinone-induced writhing model in mouse. L-arginine, a NO precursor, displayed antinociceptive effects at the doses of 0.125-1.0 mg/kg. When the doses of L-arginine were increased gradually to 10-100 mg/kg, a dose-dependent triphasic pattern of nociception-antinociception-nociception was obtained. The NO synthase (NOS) inhibitor, NC-nitro-L-arginine methyl ester (L-NAME) (18.75-150 mg/kg), possessed antinociceptive activity. Methylene blue (MB), a guanylyl cyclase and/or NOS inhibitor, (5-160 mg/kg) also produced a dose-dependent triphasic response. When L-arginine (50 mg/kg) was combined with L-NAME (75 mg/kg), L-arginine-induced antinociception did not change significantly. Cotreatment of L-arginine with 5 mg/kg MB significantly decreased MB-induced antinociception and reversed the nociception induced by 40 mg/kg MB to antinociception. It is concluded that the components of L-arginine/nitric oxide/cGMP cascade may participate in nociceptive processes both peripherally and centrally by a direct effect on nociceptors or by the involvement of other related pathways of nociceptive processes induced by NO. (C) 2000 Elsevier Science Inc. All rights reserved.