Akademik Veri Yönetim Sistemi
ACS OMEGA, cilt.8, sa.33, ss.30285-30293, 2023 (SCI-Expanded)
Carbon nanodots havedrawn a great deal of attention due to theirgreen and expedient opportunities in biological and chemical sciences.Their high fluorescence capabilities and low toxicity for living cellsand tissues make them excellent imaging agents. In addition, theyhave a fluorimetric response against inorganic and organic species.Boron-doped carbon nanodots (B-CDs) with high fluorescence yield wereproduced from phenylboronic acid and glutamine as boron and carbonsources, respectively, by a hydrothermal method. First, the effectsof the temperature on their fluorescence yield and the structuralcharacteristics of B-CDs were investigated. Second, their cytotoxicityand cell death and proliferation behaviors were examined. The cytotoxicitywas evaluated by the MTT assay. The cellular properties were evaluatedwith the distribution of caspase 3, Ki67, lamin B1, P16, and cytochrome c after the indirect immunoperoxidase technique. After theMTT assay, 1:1 dilution of all applicants for 24 h was used in thestudy. After immunohistochemical analyses, the application of B-CDssynthesized at 230 & DEG;C did not change control cell (Vero) proliferation,and also apoptosis was not triggered. Colo 320 CD133+ and CD133-cell-triggered apoptosis and cellular senescence were found to besynthesis temperature dependent. In addition, Colo 320 CD133-cells were affected relatively more than CD133+ cells from B-CDs.While B-CDs did not affect the control cells, the colon cancer stemcells (Colo 320 CD133+) were affected in a time-dependent manner.Therefore, the use of the synthesized B-CD product may be an alternativemethod for controlling or eliminating cancer stem cells in the tumortissue.