Immobilization of glucose oxidase attached to new nanospheres including azomethine

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Özdem N., Hasanoğlu Özkan E., Sarı N., Arslan F., Tümtürk H.

MACROMOLECULAR RESEARCH, vol.22, pp.1282-1287, 2014 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 22
  • Publication Date: 2014
  • Doi Number: 10.1007/s13233-014-2178-7
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.1282-1287
  • Keywords: biocatalysis, crystal, glucose oxidase, nanospheres, BIOSENSOR, GALACTOSIDASE


New nanosphere supports were prepared and characterized for the immobilization of glucose oxidase from Aspergillus niger. Nanoparticles ((APS-tio), (APS-tioCH(3)), and (APS-tioCl)) modified (aminomethyl)polystyrene (APS) with some tyophenealdehyde derivatives were synthesized by means of condensation and we investigated the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on them. Modified polystyrene was characterized by IR spectra, gel permeation chromatography and scanning electron microscopy. Immobilized GOx on (APS-tioCH(3)) showed a optimum pH, whereas immobilized GOx on (APS-tio) and (APS-tioCl) performed the optimum condition at two pHs.