The effects of resveratrol treatment on enos expression ın alloxan diabetic rabbits

Thesis Type: Post Graduate

Institution Of The Thesis: Gazi Üniversitesi, Sağlık Bilimleri Enstitüsü, Turkey

Approval Date: 2010


Consultant: FATMA AKAR


Diabetes is a metabolic disease that is known for a long time and has an increased incidence in population. Decrease in nitric oxide, released form endothelium of blood vessels is responsible for cardiovascular system diseases, that are concomitant with this metabolic disease. It has been proven that resveratrol, one of the natural phytoalexin structure compounds known to be responsible for the antioxidant effect of red wine, possesses improving effect on the nitric oxide production system. The aim of this study is to find out whether resveratrol has any protective effect on the endothelial function that is disturbed by diabetes. Diabetes was induced in rabbits by intravenously injection of 100 mg/kg alloxan. In treatment groups, resveratrol, at 5 and 50 mg/L concentrations, is administrated in drinking water for 8-10 weeks. The effect of long-term treatment with resveratrol on expression of endothelial nitric oxide synthase mRNA is determined with reverse transcriptase-polymerase chain reaction (RT-PCR). There was no significant difference between eNOS mRNA levels of the groups, after resveratrol treatment. These results indicated that beneficial effects of resveratrol did not result in increase eNOS mRNA expression. However the otlher studies with this project showed that resveratrol alleviates type 1 diabetes induced vasculopathy by decreasing vascular oxidative stress and thereby increasing the bioavailability of nitric oxide without changing metabolic abnormalities.