Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi University, Sağlık Bilimleri Enstitüsü, FARMASÖTİK TOKSİKOLOJİ ANABİLİM DALI, Turkey
Approval Date: 2021
Thesis Language: Turkish
Student: Merve Nisa Selcen GÜRBÜZ
Supervisor: Emre Durmaz
Open Archive Collection: AVESIS Open Access Collection
Abstract:
Pyrrolizidine alkaloids (PA) are the most common toxic secondary plant metabolites found in more
than 6000 plant species worldwide. These compounds especially those with 1,2 unsaturated bonds,
pose a threat to human health with their genotoxic and carcinogenic properties. There is concern and
growing awareness among regulators about the risks associated with consuming PA-contaminated
products. For this purpose a method based on quadrupole time-of-flight mass spectrometry-liquid
chromatography (LC-MS-QTOF) was validated to determine the amount of PA in herbal
supplements and teas. 28 PA were analyzed. Quantification of products such as green tea and green
tea supplements, form teas and chamomile teas, mixed herbal teas, and products containing PA
alkaloids such as comfrey and coltsfoot were performed .In our study, 11 different PAs, mostly
intermidine and N-oxide, were found in 34 different products. At least one PA was detected in 44%
(14) of 31 samples, excluding PA-containing samples. The highest total PA amounts belong to
Sympytum officinale samples, as expected. In the analyzed samples, varying amounts of PAs were
found in the range of 2.70 ng/g-9.37 μg/g in 17 of them. It was concluded that some of the amounts
detected in the samples were above the allowable limit values. The first data on different types of
samples found in our country were obtained.
Key Words : LC-MS-QTOF, Pyrrolizidine Alkaloids, herbal supplements, herbal
teas