Is cytogenetic diagnosis of 46,XX karyotype spontaneous abortion specimens erroneous? Fluorescence in situ hybridization as a confirmatory technique

Karaoguz, MERAL; Nas, T; Konac, ECE; Ince, D; Pala, E; Menevse, S

doi:10.1111/j.1447-0756.2005.00330.x

Is cytogenetic diagnosis of 46,XX karyotype spontaneous abortion specimens erroneous? Fluorescence in situ hybridization as a confirmatory technique

Atıf İçin Kopyala

Karaoguz M., Nas T., Konac E., Ince D., Pala E., Menevse S.

JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH, cilt.31, sa.6, ss.508-513, 2005 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 31 Sayı: 6
Basım Tarihi: 2005
Doi Numarası: 10.1111/j.1447-0756.2005.00330.x
Dergi Adı: JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.508-513
Gazi Üniversitesi Adresli: Evet

Özet

Aim: Performing the standard cytogenetic technique on spontaneous abortion material is still a valuable tool, but finding a normal 46,XX karyotype can confuse investigators and lead to a problem in diagnosis. This is mainly because it is possible for the female or male conceptus to retain contaminating maternal cells. To address this possibility, we used fluorescence in situ hybridization technique (FISH). X (DXZ1: p11.1-q11.1 region) and Y (DYZ3: p11.1-q11.1 region) chromosome alpha-satellite probes were employed to confirm the karyotypes previously diagnosed as 46,XX by our cytogenetic laboratory, or to verify the occurrence of 'Y chromosome component'.