Cynanchum acutum L., Cionura erecta (L.) Griseb. and Trachomitum venetum (L.) Woodson subsp. sarmatiense (Woodson)Avetisian are commonly used species in folk medicine. In the present study, the antioxidant and antimicrobial properties of the ethanol extracts of three plants were evaluated with agar well diffusion technique, the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging, reducing power, total antioxidant capacities, and total phenolic contents, respectively. Antimicrobial activities of ethanol extracts were tested against bacteria strains (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis, Bacillus cereus, Enterobacter fecalis) and two yeast strain (Candida albicans, Candida tropicalis). Minimal inhibitory concentration (MIC) values of each species were determined. All the ethanol extracts showed significant antioxidant activity. The IC(50) of ethanol extracts ranged between 196.09 and 570.71 mu g/mL. The reducing powers increased with increasing amount of extract sample. Reducing power, total antioxidant capacities of the ethanol extracts were as follows: C. acutum > C. erecta > T. venetum. The strongest antibacterial activity was observed using the ethanol extract of C. erecta against S.aureus (zone of inhibition 19.6 mm).