Optimization of fermentation conditions for the production of ethanol from sucrose by Saccharomyces cerevisiae

Tosun A.

INTERNATIONAL SUGAR JOURNAL, vol.111, no.1323, pp.181-186, 2009 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 111 Issue: 1323
  • Publication Date: 2009
  • Journal Name: INTERNATIONAL SUGAR JOURNAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.181-186
  • Keywords: cell density, design method, ethanol fermentation, optimization, Saccharomyces cerevisiae, sucrose concentration, yield, SUGAR CONCENTRATION, D-XYLOSE, TEMPERATURE, OXYGEN, XYLITOL, GROWTH
  • Gazi University Affiliated: No

Abstract

In this paper, a statistical model which can be used to describe ethanol yield per unit time from sucrose by Saccharomyces cerevisiae was developed. Effect of total sugar concentration, cell density, and shaking rate on yield per unit time were examined in repeated shake flask cultures with the use of experimental conditions designed according to the Box-Wilson experimental design method. With the use of the developed model a maximum yield per unit time of 2.2 L/h was obtained when sugar concentration, cell density, and shaking rate, were 130.5 g/L, 2.0 g/L, and 159 rpm respectively.